Characterisation and agronomic evaluation of acidified food waste anaerobic digestate products.

Raw liquid anaerobic digestate was synthesised into nutrient-dense solid digestates via acidification and evaporation. Acidification retained ammonium in the digestate whilst also donating the anion to free ammonium to form an ammonium salt. Digestate was treated with the addition of sulphuric, nitric, and phosphoric acid resulting in the formation of ammonium sulphate, ammonium nitrate and ammonium phosphate, respectively then evaporated into a solid fertiliser product. FTIR, XRD and SEM-EDS collectively confirm that the addition of acids completely converted the free ammonium in the raw digestate into their respective ammonium salt counterparts. Compounds of potassium chloride, silicon dioxide, calcium carbonate, magnesium ammonium phosphate, sodium nitrate, and sodium chloride were identified in all solid digestate samples. Plant growth and grain yield was higher in urea ammonium nitrate, raw liquid digestate and acidified digestate products compared to control and unacidified solid digestate. Urea ammonium nitrate and ammonium nitrate solid digestate had the highest dry shoot, likely due to the high available nitrogen found in both fertilisers. Overall, acidification and evaporation of liquid digestate can efficiently transform it into a valuable solid fertiliser with a high nutrient density. This process not only has the potential to mitigate handling and storage constraints of low nutrient density digestate in anaerobic digestion facilities but also offers a sustainable alternative to conventional fertilisers.

Variability in cadmium stress tolerance among four maize genotypes: Impacts on plant physiology, root morphology, and chloroplast microstructure.

Cadmium (Cd) is detrimental to both plants and humans. Maize (Zea mays L.) genotypes exhibit variations in Cd accumulations. This study examined variations in Cd accumulation and tolerance among four maize genotypes with contrasting root morphology. The four maize genotypes were cultivated in a semi-hydroponic system with three Cd concentrations (0, 10, 20 µmol L-1). The effects of Cd on plant growth and physiology were assessed 39 days after transplanting. Results showed that root characteristics were positively correlated with root Cd accumulation and the bioconcentration factor under Cd20 treatment. Genotypes Shengrui999 and Zhengdan958 exhibited higher total Cd content than Xundan29 and Zhongke11 under Cd20 conditions. Cd toxicity led to membrane degradation of chloroplast mesophyll cells, loosening and swelling of grana lamella, and reduced starch reserves. The greater tolerance of Shengrui999 and Zhengdan958 was contributed to factors such as root biomass, shallower root depth, higher Cd content, accumulation of osmolyte such as soluble protein, antioxidant activities such as catalase (CAT), and the presence of phytohormone gibberellic acid. The study establishes a link between root morphology, Cd accumulation, and tolerance in maize plants, as demonstrated by the higher Cd accumulation and shallower root system in Cd-tolerant genotypes. This research provides a foundation for breeding maize cultivars better suited for adaptation to moderate Cd-contaminated environments.

Enhancing nutrient recovery from food waste anaerobic digestate.

The study synthesised the raw liquid fraction of digestate into a nutrient rich solid digestate through acidification whilst preventing nitrogen loss through ammonium volatilisation during evaporation. To stabilise ammonium in the digestate, it was acidified with sulphuric, nitric, and phosphoric acid to produce solid digestate with ammonium sulphate, ammonium nitrate and ammonium phosphate, respectively. These treatments were compared against urea ammonium nitrate, raw digestate, and unacidified solid digestate. To evaluate the effect of these transformed digestate products in soil, a plant growth experiment (Kikuyu; Cenchrus clandestinus) was conducted, and characterised, plant growth, soil chemistry, and rhizosphere bacterial communities. Plant growth was enhanced by all digestate treatments compared to control and urea ammonium nitrate. Ammonium phosphate solid digestate plant growth was significantly higher than all other acidified treatments due to the high P content. Moreover, digestate-amended soil had elevated Proteobacteria and putative denitrification genes.

Nitrogen dynamics and biological processes in soil amended with microalgae grown in abattoir digestate to recover nutrients.

The use of microalgae for nutrient recovery from wastewater and subsequent conversion of the harvested biomass into fertilizers offers a sustainable approach towards creating a circular economy. Nonetheless, the process of drying the harvested microalgae represents an additional cost, and its impact on soil nutrient cycling compared to wet algal biomass is not thoroughly understood. To investigate this, a 56-day soil incubation experiment was conducted to compare the effects of wet and dried Scenedesmus sp. microalgae on soil chemistry, microbial biomass, CO2 respiration, and bacterial community diversity. The experiment also included control treatments with glucose, glucose + ammonium nitrate, and no fertilizer addition. The Illumina Mi-Seq platform was used to profile the bacterial community and in-silico analysis was performed to assess the functional genes involved in N and C cycling processes. The maximum CO2 respiration and microbial biomass carbon (MBC) concentration of dried microalgae treatment were 17% and 38% higher than those of paste microalgae treatment, respectively. NH4+ and NO3- released slowly and through decomposition of microalgae by soil microorganisms as compared to synthetic fertilizer control. The results indicate that heterotrophic nitrification may contribute to nitrate production for both microalgae amendments, as evidenced by low amoA gene abundance and a decrease in ammonium with an increase in nitrate concentration. Additionally, dissimilatory nitrate reduction to ammonium (DNRA) may be contributing to ammonium production in the wet microalgae amendment, as indicated by an increase in nrfA gene and ammonium concentration. This is a significant finding because DNRA leads to N retention in agricultural soils instead of N loss via nitrification and denitrification. Thus, further processing the microalgae through drying or dewetting may not be favorable for fertilizer production as the wet microalgae appeared to promote DNRA and N retention.

Combining frass and fatty acid co-products derived from Black soldier fly larvae farming shows potential as a slow release fertiliser.

Use of black soldier fly larvae (BSFL) to process large volumes of organic waste is an emerging industry to produce protein. A co-product of this industry, the larval faeces (frass), has potential to be used as an organic fertiliser in a circular economy. However, BSFL frass has a high ammonium (N-NH4+) content which could result in nitrogen (N) loss following its application to land. One solution is to process the frass by combining it with solid fatty acids (FA) that have previously been used to manufacture slow-release inorganic fertilisers. We investigated the slow-releasing effect of N after combining BSFL frass with three FAs - lauric, myristic and stearic acid. Soil was amended with the three forms of FA processed (FA-P) frass, unprocessed frass or a control and incubated for 28 days. The impact of treatments on soil properties and soil bacterial communities were characterised during the incubation. Lower N-NH4+ concentrations occurred in soil treated with FA-P frass compared to unprocessed frass, and N-NH4+ release was slowest for lauric acid processed frass. Initially, all frass treatments caused a large shift in the soil bacterial community towards a dominance of fast-growing r-strategists that were correlated with increased organic carbon levels. FA-P frass appeared to enhance the immobilisation of N-NH4+ (from frass) by diverting it into microbial biomass. Unprocessed and stearic acid processed frass became enriched by slow-growing K-strategist bacteria at the latter stages of the incubation. Consequently, when frass was combined with FAs, FA chain length played an important role in regulating the composition of r-/K- strategists in soil and N and carbon cycling. Modifying frass with FAs could be developed into a slow release fertiliser leading to reduced soil N loss, improved fertiliser use efficiency, increased profitability and lower production costs.

Development of a controlled release fertilizer by incorporating lauric acid into microalgal biomass: Dynamics on soil biological processes for efficient utilisation of waste resources.

Utilisation of microalgae to extract nutrients from the effluent of anaerobic digestion of food waste is an emerging technology. A by-product of this process is the microalgal biomass which has potential to be used as an organic bio-fertilizer. However, microalgal biomass are rapidly mineralized when applied to soil which may result in N loss. One solution is to emulsify microalgal biomass with lauric acid (LA) to delay the release of mineral N. This study aimed to investigate whether combining LA with microalgae to develop a new fertilizer product with a controlled release function of mineral N when applied to soil, and any potential impacts the bacterial community structure and activity. The treatments were applied to soil emulsified with LA and were combined with either microalgae or urea at rates of 0%, 12.5%, 25% and 50% LA, untreated microalgae or urea and unamended control were incubated at 25 °C and 40% water holding capacity for 28 days. Quantification of soil chemistry (NH4+-N, NO3--N, pH and EC), microbial biomass carbon, CO2 production and bacterial diversity were characterised at 0, 1, 3, 7, 14 and 28 days. The NH4+-N and NO3--N concentration decreased with increasing rate of LA combined microalgae indicating that both N mineralization and nitrification were impacted. As a function of time, NH4+-N concentration increased up to 7 days for the microalgae at lower rates of LA, and then slowly decreased for 14 and 28 days, with an inverse relationship with soil NO3-N. Aligning with soil chemistry, an observed decrease in the predicted nitrification genes amoA·amoB and relative abundance of ammonia oxidizing bacteria (Nitrosomonadaceae) and nitrifying bacteria (Nitrospiraceae) with an increasing rate of LA with microalgae provides further support for possible inhibition of nitrification. The MBC and CO2 production was higher in the soil amended with increasing rates of LA combined microalgae and there was an increase in the relative abundance of fast-growing heterotrophs. Treating microalgae by emulsification with LA has the potential to control the release of N by increasing immobilization over nitrification and therefore it might be possible to engineer microalgae to match plant nutrient growth requirements whilst recovering waste from waste resources.

Lactic acid production from food waste at an anaerobic digestion biorefinery: effect of digestate recirculation and sucrose supplementation.

Low lactic acid (LA) yields from direct food waste (FW) fermentation restrict this production pathway. However, nitrogen and other nutrients within FW digestate, in combination with sucrose supplementation, may enhance LA production and improve feasibility of fermentation. Therefore, this work aimed to improve LA fermentation from FWs by supplementing nitrogen (0-400 mgN·L-1) as NH4Cl or digestate and dosing sucrose (0-150 g·L-1) as a low-cost carbohydrate. Overall, NH4Cl and digestate led to similar improvements in the rate of LA formation (0.03 ± 0.02 and 0.04 ± 0.02 h-1 for NH4Cl and digestate, respectively), but NH4Cl also improved the final concentration, though effects varied between treatments (5.2 ± 4.6 g·L-1). While digestate altered the community composition and increased diversity, sucrose minimised community diversion from LA, promoted Lactobacillus growth at all dosages, and enhanced the final LA concentration from 25 to 30 g·L-1 to 59-68 g·L-1, depending on nitrogen dosage and source. Overall, the results highlighted the value of digestate as a nutrient source and sucrose as both community controller and means to enhance the LA concentration in future LA biorefinery concepts.

Impacts of Rock Mineral and Traditional Phosphate Fertilizers on Mycorrhizal Communities in Pasture Plants.

Intensive fertilizer use can constrain contributions from soil biological processes in pastures, including those associated with arbuscular mycorrhizal (AM) fungi. We evaluated the effect of fertilizers of different P solubility on the colonization of the roots of two common pasture plants by a community of AM fungi in a pasture soil. The treatments were a rock mineral fertilizer, a chemical fertilizer and a microbial inoculant. Subterranean clover and annual ryegrass were grown in pots for 10 weeks. Both fertilizers reduced the proportion and length of roots colonized by naturally occurring AM fungi. However, by 10 weeks, there was a much greater length of mycorrhizal root for annual ryegrass than for subterranean clover. The relative abundance of mycorrhizal fungi in the families Glomeraceae and Acaulosporaceae in roots was not affected by the form of fertilizer, but diversity indices of AM fungi in roots were altered. The chemical fertilizer had a greater negative effect on AM fungal diversity indices in the annual ryegrass roots compared with the subterranean clover roots. The reduction in OTU richness of AM fungi with fertilizer application corresponded with reduced soil pH. Differential effects of P fertilizers on naturally occurring AM fungi in this agricultural soil have the potential to influence the efficacy of P fertilizer use and dominance of plant species in grasslands.

Environmental implications, potential value, and future of food-waste anaerobic digestate management: A review.

Globally, the valorisation of food waste into digestate through the process of anaerobic digestion is becoming increasingly popular. As a result, a large amount of food-waste digestate will need to be properly utilised. The utilisation of anaerobic digestion for fertiliser and alternative uses is essential to obtain a circular bioeconomy. The review aims to examine the environmental management of food-waste digestate, the value of digestate as a fertiliser and soil conditioner, and the emerging uses and improvements for post-anaerobic digestion reuse of digestate. Odour emissions, contaminants in food waste, emission and leaching of nutrients into the environment, and the regulations, policies, and voluntary initiatives of anaerobic digestion are evaluated in the review. Food-waste digestate can provide essential nutrients, carbon, and bio-stimulants to soils and increase yield. Recently, promising research has shown that digestates can be used in hydroponic systems and potentially replace the use of synthetic fertilisers. The integration of anaerobic digestion with emerging uses, such as extraction of value-added products, algae cultivation, biochar and hydrochar production, can further reduce inhibitory sources of digestate and provide additional economic opportunities for businesses. Moreover, the end-product digestate from these technologies can also be more suitable for use in soil application and hydroponic use.

Physical, chemical, and microbial contaminants in food waste management for soil application: A review.

Currently, 1.3 billion tonnes of food are thrown away each year, most of which are incinerated or landfilled causing large environmental, social, and economic issues. Therefore, the utilisation of food waste as biofertilisers, such as composts and digestates, is a solution to reduce the problems created by incineration and landfilling whilst simultaneously amending soils. The improper disposal of food wastes and bulking materials can contribute to high levels of contaminants within the end-product. Moreover, the food waste and bulking materials, themselves, may contain trace amounts of contaminants. These contaminants tend to have long half-lives, are easily mobile within soil and plants, can accumulate within the food supply chain, and have moderate to high levels of toxicity. This review aims to examine the current and emerging contaminants of high concern that impact the quality of food-waste fertilisers. The paper presents the volume of current and emerging contaminants of plastics, other physical (particulate) contaminants, heavy metals, pesticides, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), per- and polyfluoroalkyl substances (PFAS), and pathogens within food-waste composts and digestates. Due to the large extent of organic chemical contaminants and the unknown level of toxicity and persistence, the risk assessment of organic chemical contaminants in the food-supply chain remains largely unknown. This study has presented available data from literature of various contaminants found in food waste, and composts and digestates derived from food waste, and evaluated the data with current regulations globally. Overall, to reduce contaminants in composts and digestates, more studies are required on the implementation of proper disposal separation, effective composting and digestion practices, increased screening of physical contaminants, development of compostable plastics, and increased regulatory policies on emerging, problematic contaminants. Moreover, examination of emerging contaminants in food-waste composts and digestates is needed to ensure food security and reduce future human-health risks.

Treatment processes to eliminate potential environmental hazards and restore agronomic value of sewage sludge: A review.

Land application of sewage sludge is increasingly used as an alternative to landfilling and incineration owing to a considerable content of carbon and essential plant nutrients in sewage sludge. However, the presence of chemical and biological contaminants in sewage sludge poses potential dangers; therefore, sewage sludge must be suitably treated before being applied to soils. The most common methods include anaerobic digestion, aerobic composting, lime stabilization, incineration, and pyrolysis. These methods aim at stabilizing sewage sludge, to eliminate its potential environmental pollution and restore its agronomic value. To achieve best results on land, a comprehensive understanding of the transformation of organic matter, nutrients, and contaminants during these sewage-sludge treatments is essential; however, this information is still lacking. This review aims to fill this knowledge gap by presenting various approaches to treat sewage sludge, transformation processes of some major nutrients and pollutants during treatment, and potential impacts on soils. Despite these treatments, overtime there are still some potential risks of land application of treated sewage sludge. Potentially toxic substances remain the main concern regarding the reuse of treated sewage sludge on land. Therefore, further treatment may be applied, and long-term field studies are warranted, to prevent possible adverse effects of treated sewage sludge on the ecosystem and human health and enable its land application.

Alterations to arbuscular mycorrhizal fungal community composition is driven by warming at specific elevations.

BACKGROUND: Global warming can alter plant productivity, and community composition which has consequences for soil-plant associated microorganisms. Arbuscular mycorrhizal fungi (AMF) are distributed widely and form symbiotic relationships with more than 80% of vascular plants and play a key role in nutrient cycling processes at the ecosystem scale. METHODS: A simulated warming experiment at multiple elevations (3,000, 3,500, 3,800, and 4,170 m) was conducted utilizing an in-situ open-top chamber (OTC) for exploring the effect of global warming on AMF community structure in the Qinghai-Tibet Plateau (QTP). This region has been identified as one of the most sensitive areas to climatic changes. Soil DNA was extracted and sequenced using next the Mi-Seq platform for diversity profiling. RESULTS: AMF richness was higher under the simulated warming chamber, however this only occurred in the elevation of 3,500 m. Warming did not alter other AMF alpha diversity indices (e.g. Shannon, Ace, and Simpson evenness index). Glomus and Acaulospora were the dominate AMF genera as assessed through their relative abundance and occurrence in control and warming treatments at the different elevations. CONCLUSION: Warming changed significantly AMF community. The effects of warming on AMF community structure varied depend on elevations. Moreover, the occurrences of AMF in different genera were also presented the different responses to warming in four elevations.

Co-application of a biosolids product and biochar to two coarse-textured pasture soils influenced microbial N cycling genes and potential for N leaching.

Co-application of biochar and biosolids to soil has potential to mitigate N leaching due to physical and chemical properties of biochar. Changes in N cycling pathways in soil induced by co-application of biological amendments could further mitigate N loss, but this is largely unexplored. The aim of this study was to determine whether co-application of a biochar and a modified biosolids product to three pasture soils differing in texture could alter the relative abundance of N cycling genes in soil sown with subterranean clover. The biosolids product contained lime and clay and increased subterranean clover shoot biomass in parallel with increases in soil pH and soil nitrate. Its co-application with biochar similarly increased plant growth and soil pH with a marked reduction in nitrate in two coarse textured soils but not in a clayey soil. While application of the biosolids product altered in silico predicted N cycling functional genes, there was no additional change when applied to soil in combination with biochar. This supports the conclusion that co-application of the biochar and biosolids product used here has potential to mitigate loss of N in coarse textured soils due to N adsoption by the biochar and independently of microbial N pathways.

Plant-Dependent Soil Bacterial Responses Following Amendment With a Multispecies Microbial Biostimulant Compared to Rock Mineral and Chemical Fertilizers.

Biostimulants are gaining momentum as potential soil amendments to increase plant health and productivity. Plant growth responses to some biostimulants and poorly soluble fertilizers could increase soil microbial diversity and provide greater plant access to less soluble nutrients. We assessed an agricultural soil amended with a multispecies microbial biostimulant in comparison with two fertilizers that differed in elemental solubilities to identify effects on soil bacterial communities associated with two annual pasture species (subterranean clover and Wimmera ryegrass). The treatments applied were: a multispecies microbial biostimulant, a poorly soluble rock mineral fertilizer at a rate of 5.6 kg P ha-1, a chemical fertilizer at a rate of 5.6 kg P ha-1, and a negative control with no fertilizer or microbial biostimulant. The two annual pasture species were grown separately for 10 weeks in a glasshouse with soil maintained at 70% of field capacity. Soil bacteria were studied using 16S rRNA with 27F and 519R bacterial primers on the Mi-seq platform. The microbial biostimulant had no effect on growth of either of the pasture species. However, it did influence soil biodiversity in a way that was dependent on the plant species. While application of the fertilizers increased plant growth, they were both associated with the lowest diversity of the soil bacterial community based on Fisher and Inverse Simpson indices. Additionally, these responses were plant-dependent; soil bacterial richness was highly correlated with soil pH for subterranean clover but not for Wimmera ryegrass. Soil bacterial richness was lowest following application of each fertilizer when subterranean clover was grown. In contrast, for Wimmera ryegrass, soil bacterial richness was lowest for the control and rock mineral fertilizer. Beta diversity at the bacterial OTU level of resolution by permanova demonstrated a significant impact of soil amendments, plant species and an interaction between plant type and soil amendments. This experiment highlights the complexity of how soil amendments, including microbial biostimulants, may influence soil bacterial communities associated with different plant species, and shows that caution is required when linking soil biodiversity to plant growth. In this case, the microbial biostimulant influenced soil biodiversity without influencing plant growth.

Microalgae and Phototrophic Purple Bacteria for Nutrient Recovery From Agri-Industrial Effluents: Influences on Plant Growth, Rhizosphere Bacteria, and Putative Carbon- and Nitrogen-Cycling Genes.

Microalgae (MA) and purple phototrophic bacteria (PPB) have the ability to remove and recover nutrients from digestate (anaerobic digestion effluent) and pre-settled pig manure that can be Utilized as bio-fertilizer and organic fertilizer. The objective of this study was to compare the effectiveness of MA and PPB as organic fertilizers and soil conditioners in relation to plant growth and the soil biological processes involved in nitrogen (N) and carbon (C) cycling. To this end, a glasshouse experiment was conducted using MA and PPB as bio-fertilizers to grow a common pasture ryegrass (Lolium rigidum Gaudin) with two destructive harvests (45 and 60 days after emergence). To evaluate the rhizosphere bacterial community, we used barcoded PCR-amplified bacterial 16S rRNA genes for paired-end sequencing on the Illumina Mi-Seq. Additionally, we used phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis for the detection of putative functional genes associated with N and soil-C cycling. There was a significant increase in plant growth when the soil was amended with PPB, which almost performed as well as the chemical fertilizers. Analysis of the rhizosphere bacteria after the second harvest revealed a greater abundance of Firmicutes than in the first harvest. Members of this phylum have been identified as a biostimulant for plant growth. In contrast, the MA released nutrients more slowly and had a profound effect on N cycling by modulating N mineralization and N retention pathways. Thus, MA could be developed as a slow-release fertilizer with better N retention, which could improve crop performance and soil function, despite nutrient losses from leaching, runoff, and atmospheric emissions. These data indicate that biologically recovered nutrients from waste resources can be effective as a fertilizer, resulting in enhanced C- and N-cycling capacities in the rhizosphere.

Soil Salinity and pH Drive Soil Bacterial Community Composition and Diversity Along a Lateritic Slope in the Avon River Critical Zone Observatory, Western Australia.

Soils are crucial in regulating ecosystem processes, such as nutrient cycling, and supporting plant growth. To a large extent, these functions are carried out by highly diverse and dynamic soil microbiomes that are in turn governed by numerous environmental factors including weathering profile and vegetation. In this study, we investigate geophysical and vegetation effects on the microbial communities of iron-rich lateritic soils in the highly weathered landscapes of Western Australia (WA). The study site was a lateritic hillslope in southwestern Australia, where gradual erosion of the duricrust has resulted in the exposure of the different weathering zones. High-throughput amplicon sequencing of the 16S rRNA gene was used to investigate soil bacterial community diversity, composition and functioning. We predicted that shifts in the microbial community would reflect variations in certain edaphic properties associated with the different layers of the lateritic profile and vegetation cover. Our results supported this hypothesis, with electrical conductivity, pH and clay content having the strongest correlation with beta diversity, and many of the differentially abundant taxa belonging to the phyla Actinobacteria and Proteobacteria. Soil water repellence, which is associated with Eucalyptus vegetation, also affected beta diversity. This enhanced understanding of the natural system could help to improve future crop management in WA since the physicochemical properties of the agricultural soils in this region are inherited from laterites via the weathering and pedogenesis processes.

Ammonia stress on a resilient mesophilic anaerobic inoculum: Methane production, microbial community, and putative metabolic pathways.

Short term inhibition tests, 16S rRNA tag sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), were employed to visualise the effects of increasing total ammoniacal nitrogen (TAN) concentration (3400-10166 ppm TAN) on microbial community structure and metabolic pathways for acetate degradation. The rate of methane production on acetate was significantly reduced by TAN concentrations above 6133 ppm; however, methane continued to be produced, even at 10166 ppm TAN (0.026 ±â€¯0.0003 gCOD.gVS-1inoculum.day-1). Hydrogenotrophic methanogenesis with syntrophic acetate oxidation (SAO) was identified as the dominant pathway for methane production. A shift towards SAO pathways at higher TAN concentrations and a decrease in the number of 'gene hits' for key genes in specific methanogenesis pathways was observed. Overall, the results highlighted potential for inhibition activity testing to be used together with PICRUSt, to estimate changes in microbial metabolism and to better understand microbial resilience in industrial AD facilities.

Response of Wheat to a Multiple Species Microbial Inoculant Compared to Fertilizer Application.

Microbial inoculants, including those formed from multiple species, may have dual functions as biostimulants and/or biocontrol agents, and claimed agricultural benefits are instrumental for regulatory categorisation. Biostimulants include commercial products containing substances or microorganisms that stimulate plant growth. Biostimulant microbes can be involved in a range of processes that affect N and P transformations in soil and thus influence nutrient availability, and N and P fertilizers can influence soil microbial diversity and function. A glasshouse experiment was conducted to investigate the effect of a multiple species microbial inoculant relative to a rock-based mineral fertilizer and a chemical fertilizer on wheat growth and yield, and on microbial diversity in the rhizosphere. The microbial inoculant was compared to the mineral fertilizer (equivalent to 5.6 kg N ha-1 and 5.6 kg P ha-1), and to the chemical fertilizer applied at three rates equivalent to: (i) 7.3 kg N ha-1 and 8.4 kg P ha-1 as recommended for on-farm use, (ii) 5.6 kg N ha-1 and 6.5 kg P ha-1 which matched the N in the mineral fertilizer, and (iii) 4.9 kg N ha-1 and 5.6 kg P ha-1 which matched P content in the mineral fertilizer. Despite an early reduction in plant growth, the microbial inoculant treatment increased shoot growth at maturity compared to the control. Similarly, grain yield was higher after application of the microbial inoculant when compared to control, and it was similar to that of plants receiving the fertilizer treatments. Using 16S rRNA sequencing, the microbial inoculant and fertilizer treatments were shown to influence the diversity of rhizosphere bacteria. The microbial inoculant increased the relative abundance of the phylum Actinobacteria. At tillering, the proportion of roots colonized by arbuscular mycorrhizal (AM) fungi increased with the microbial inoculant and mineral fertilizer treatments, but decreased with the chemical fertilizer treatments. At maturity, there were no treatment effects on the proportion of wheat roots colonized by AM fungi. Overall, the multiple species microbial inoculant had beneficial effects in terms of wheat yield relative to the commercial mineral and chemical fertilizers applied at the level recommended for on-farm use in south-western Australia.

Interactions between biochar and mycorrhizal fungi in a water-stressed agricultural soil.

Biochar may alleviate plant water stress in association with arbuscular mycorrhizal (AM) fungi but research has not been conclusive. Therefore, a glasshouse experiment was conducted to understand how interactions between AM fungi and plants respond to biochar application under water-stressed conditions. A twin chamber pot system was used to determine whether a woody biochar increased root colonisation by a natural AM fungal population in a pasture soil ('field' chamber) and whether this was associated with increased growth of extraradical AM fungal hyphae detected by plants growing in an adjacent ('bait') chamber containing irradiated soil. The two chambers were separated by a mesh that excluded roots. Subterranean clover was grown with and without water stress and harvested after 35, 49 and 63 days from each chamber. When biochar was applied to the field chamber under water-stressed conditions, shoot mass increased in parallel with mycorrhizal colonisation, extraradical hyphal length and shoot phosphorus concentration. AM fungal colonisation of roots in the bait chamber indicated an increase in extraradical mycorrhizal hyphae in the field chamber. Biochar had little effect on AM fungi or plant growth under well-watered conditions. The biochar-induced increase in mycorrhizal colonisation was associated with increased growth of extraradical AM fungal hyphae in the pasture soil under water-stressed conditions.